Pulsed Laser Ablation Synthesis of TiO₂ Nanoparticles and their Enhanced Antimicrobial Activity under Femtosecond Laser Irradiation

Muhammad F. Mahdi; Esraa Ahmed; Fatma Abdel Samad; Yasmin Abd El-Salam; Mansour Hassan; Ahmed O. El-Gendy; Tarek Mohamed

doi:10.48084/etasr.15159

Authors

Muhammad F. Mahdi Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt | Anbar Health Department, Ministry of Health, Ramadi Teaching Hospital, Iraq
Esraa Ahmed Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt
Fatma Abdel Samad Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt
Yasmin Abd El-Salam Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt
Mansour Hassan Department of Ophthalmology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
Ahmed O. El-Gendy Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt | Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt
Tarek Mohamed Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, Egypt | Department of Engineering, Faculty of Advanced Technology and Multidiscipline, Universitas Airlangga, Indonesia

Volume: 16 | Issue: 2 | Pages: 32810-32817 | April 2026 | https://doi.org/10.48084/etasr.15159

Received: 26 September 2025 | Revised: 3 November 2025 and 21 November 2025 | Accepted: 24 November 2025 | Online: 4 February 2026

Corresponding author: Ahmed O. El-Gendy

Abstract

This study analyzes the synthesis of titanium dioxide TiO₂-Nanoparticles (NPs), through Pulsed Laser Ablation in Liquid (PLAL). The combination was performed by irradiation of a titanium target at its second harmonic (532 nm, 10 ns pulse duration, and 10 Hz repetition rate), bv a Nd:YAG laser. The results of the analysis verified the antibacterial efficacy of the synthesized TiO₂-NPs against Methicillin-Resistant Staphylococcus Aureus (MRSA) and Pseudomonas aeruginosa, by reducing the bacterial growth, proving an effective strategy against ani-biotic resistant pathogens.

Keywords:

femtosecond laser-based treatment, titanium dioxide nanoparticles (TiO₂-NPs), laser ablation synthesis technique

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